Rabbit ABL2 Polyclonal Antibody | anti-ABL2 antibody

Anti-ABL2 Rabbit pAb

Cat. Num. AAA4750342

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Chromatography
• Synonyms: ABL2; Polyclonal Antibody; Anti-ABL2 Rabbit pAb; ABLL; Tyrosine kinase ARG; kinase Arg; anti-ABL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABL2 Antibody detects endogenous levels of total ABL2
• Purity/Purification: Affinity Chromatography
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1064

• Applicable Applications for anti-ABL2 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; FC/FACS: 1:50
• Immunogen: A synthesized peptide derived from human ABL2
• Subcellular Location: Cell projection; Lamellipodium
• Preparation and Storage: Store at 4 degree C for short term.; Store at-20 degree C for long term.; Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of ABL2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.)

Related Product Information for anti-ABL2 antibody

ABL2 is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length.

NCBI and Uniprot Product Information

• NCBI GI #: 209862772
• NCBI GeneID: 27
• NCBI Accession #: NP_001129472.1
• NCBI GenBank Nucleotide #: NM_001136000.2
• UniProt Accession #: P42684
• Molecular Weight: 128kDa
• NCBI Official Full Name: tyrosine-protein kinase ABL2 isoform d
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: tyrosine-protein kinase ABL2; Abelson tyrosine-protein kinase 2
• UniProt Protein Name: Tyrosine-protein kinase ABL2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.