ABL1 antibody pair

ABL1 Antibody Pair

Cat. Num. AAA7041848

• Gene Names: ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• Reactivity: Bovine
• Applications: ELISA
• Synonyms: ABL1; Polyclonal Antibody Pair; ABL1 Antibody Pair; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL; JTK7; ABL1 antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Bovine
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 115

• Applicable Applications for ABL1 antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.1ug/ml and the detection antibody at a concentration of 0.1ug/ml.
• Immunogen Species: Human
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: K-562 cell; MOLT-4 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 553164
• NCBI GeneID: 25
• Molecular Weight: 124,955 Da
• NCBI Official Full Name: ABL1, partial
• NCBI Official Synonym Full Names: ABL proto-oncogene 1, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL1
• NCBI Official Synonym Symbols: ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
• NCBI Protein Information: tyrosine-protein kinase ABL1
• UniProt Protein Name: Tyrosine-protein kinase ABL1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ABL1
• UniProt Synonym Gene Names: ABL; JTK7
• UniProt Entry Name: ABL1_HUMAN

NCBI Description

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]

Uniprot Description

Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.

Protein type: Abl family; EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: actin monomer binding; ATP binding; magnesium ion binding; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein kinase C binding; protein-tyrosine kinase activity; receptor binding; SH3 domain binding; syntaxin binding

Biological Process: actin cytoskeleton organization and biogenesis; cell cycle arrest; DNA damage induced protein phosphorylation; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; elevation of cytosolic calcium ion concentration; establishment of protein localization; innate immune response; mismatch repair; mitochondrial depolarization; mitosis; negative regulation of ubiquitin-protein ligase activity; peptidyl-tyrosine phosphorylation; positive regulation of apoptosis; positive regulation of muscle cell differentiation; positive regulation of oxidoreductase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of autophagy; regulation of axon extension; regulation of cell adhesion; regulation of cell proliferation; regulation of endocytosis; regulation of microtubule polymerization; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to oxidative stress

Disease: Leukemia, Chronic Myeloid