Rabbit anti-Mouse, Rat ABCG2 Polyclonal Antibody | anti-ABCG2 antibody

ABCG2 (Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, CD338) (FITC)

Cat. Num. AAA6404086

• Gene Names: Abcg2; MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ABCG2; Polyclonal Antibody; ABCG2 (Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD338) (FITC); anti-ABCG2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse ABCG2. Species Crossreactivity: rat. Does not crossreact with other proteins.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ABCG2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a sequence, aa152-167 KNHEKNERINTIIKEL, in the middle region of mouse ABCG2. UniProt Accession: Q7TMS5, different from the related rat sequence by one amino acid.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using 144108. All lanes: 144108 (0.5ug/ml) Lane 1: Rat Liver Tissue Lysate at 50ug Lane 2: Rat Spleen Tissue Lysate at 50ug Lane 3: Rat Kidney Tissue Lysate at 50ug Lane 4: Mouse Spleen Tissue Lysate at 50ug Predicted band size: 72kD Observed band size: 72kD)

Related Product Information for anti-ABCG2 antibody

ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded that ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels. Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

Product Categories/Family for anti-ABCG2 antibody

Antibodies; Multi Drug Resistance (MDR)

References

1. Jonker, J. W., et al., The breast cancer resistance protein BCRP (ABCG2) concentrates drugs and carcinogenic xenotoxins into milk. Nature Med. 11:127-129, 2005. 2. Matsuo, H., et al., Common defects of ABCG2, a high-capacity urate exporter, cause gout: a function-based genetic analysis in a Japanese population.Sci. Transl. Med. 1:5ra11, 2009. 3. Ozvegy, C., et al., Functional characterization of the human multidrug transporter, ABCG2, expressed in insect cells.Biochem. Biophys. Res. Commun. 285:111-117, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 68052328
• NCBI GeneID: 26357
• UniProt Accession #: Q7TMS5; Q9R004; Q9Z1T0
• Molecular Weight: 72,978 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 2
• NCBI Official Symbol: Abcg2
• NCBI Official Synonym Symbols: MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558
• NCBI Protein Information: ATP-binding cassette sub-family G member 2; urate exporter; mitoxantrone resistance protein 1; breast cancer resistance protein 1 homolog
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: Abcg2
• UniProt Synonym Gene Names: Abcp; Bcrp1
• UniProt Entry Name: ABCG2_MOUSE