Immunofluorescence (IF)
(MBS9605272 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit ABCF2 Polyclonal Antibody | anti-ABCF2 antibody
ABCF2 Antibody
Gene Names
ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ABCF2; Polyclonal Antibody; ABCF2 Antibody; 0710005O05Rik; ABC28; ABCF 2; ATP binding cassette sub family F (GCN20) member 2; DKFZp586K1823; Drr3; E430001O06; EST133090; fb04f06; fc74f03; fd58d07; HUSSY 18; MABC1; MGC128735; MGC53054; wu:fb04f06; wu:fc74f03; wu:fd58d07; anti-ABCF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ABCF2 antibody detects endogenous levels of total ABCF2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
634
Applicable Applications for anti-ABCF2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Chicken (91%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605272 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605272 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ABCF2 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ABCF2 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ABCF2 antibody
Function: Lacks transmembrane domains and is probably not involved in transport.
Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 71 kD
Predicted: 72 kDa
Predicted: 72 kDa
NCBI Official Full Name
ATP-binding cassette sub-family F member 2 isoform b
NCBI Official Synonym Full Names
ATP binding cassette subfamily F member 2
NCBI Official Symbol
ABCF2
NCBI Official Synonym Symbols
ABC28; HUSSY18; HUSSY-18; EST133090
NCBI Protein Information
ATP-binding cassette sub-family F member 2
UniProt Protein Name
ATP-binding cassette sub-family F member 2
Protein Family
UniProt Gene Name
ABCF2
NCBI Description
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7. [provided by RefSeq, May 2017]
Uniprot Description
CautionLacks transmembrane domains and is probably not involved in transport.
Research Articles on ABCF2
Similar Products
Product Notes
The ABCF2 abcf2 (Catalog #AAA9605272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABCF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABCF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ABCF2 abcf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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