Rabbit AATF Polyclonal Antibody | anti-AATF antibody

AATF (N16) Polyclonal Antibody

Cat. Num. AAA3002208

• Gene Names: AATF; DED; BFR2; CHE1; CHE-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AATF; Polyclonal Antibody; AATF (N16) Polyclonal Antibody; Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; CHE1; DED; HSPC277; anti-AATF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AATF (N16) polyclonal antibody detects endogenous levels of AATF protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 560

• Applicable Applications for anti-AATF antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 10-60 of Human AATF.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AATF (N16) polyclonal antibody at 1:500 dilutionLane1:H1792 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Related Product Information for anti-AATF antibody

Che-1 (protein AATF, apoptosis-antagonizing transcription factor) is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, Che-1 may function as a general inhibitor of the histone deacetylase HDAC1. Che-1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che-1. It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer disease (AD; presenile and senile dementia). PAR4 is a leucine zipper protein that is pro-apoptotic and associated with neuronal degeneration in AD. Che-1 interaction with PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity. Che-1 also co-localizes with PAR4 in both cytoplasmic and nuclear compartments, and it interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.

NCBI and Uniprot Product Information

• NCBI GI #: 7657013
• NCBI GeneID: 26574
• NCBI Accession #: NP_036270.1
• NCBI GenBank Nucleotide #: NP_036270.1
• UniProt Accession #: Q9NY61
• Molecular Weight: ~ 63kDa
• NCBI Official Full Name: protein AATF
• NCBI Official Synonym Full Names: apoptosis antagonizing transcription factor
• NCBI Official Symbol: AATF
• NCBI Official Synonym Symbols: DED; BFR2; CHE1; CHE-1
• NCBI Protein Information: protein AATF
• UniProt Protein Name: Protein AATF
• Protein Family: Protein
• UniProt Gene Name: AATF
• UniProt Synonym Gene Names: CHE1; DED
• UniProt Entry Name: AATF_HUMAN

NCBI Description

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

AATF: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. Belongs to the AATF family.

Protein type: RNA-binding; Nucleolus; Transcription factor

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi apparatus; centrosome; focal adhesion; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; leucine zipper domain binding; transcription factor activity; tau protein binding

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of amyloid precursor protein biosynthetic process; regulation of mitotic cell cycle; ribosome biogenesis and assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of superoxide release; cell adhesion; response to DNA damage stimulus; embryonic cleavage; negative regulation of apoptosis