Immunoprecipitation (IP)
(AARSD1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-AARSD1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AARSD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 45 kDa)
Rabbit anti-Human AARSD1 Polyclonal Antibody | anti-AARSD1 antibody
Anti-AARSD1 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(AARSD1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-AARSD1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AARSD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 45 kDa)
Immunoprecipitation (IP)
(AARSD1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-AARSD1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AARSD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 45 kDa)
Western Blot (WB)
(Anti-AARSD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-AARSD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
This locus represents naturally occurring readthrough transcription between the neighboring PTGES3L (prostaglandin E synthase 3 (cytosolic)-like) and AARSD1(alanyl-tRNA synthetase domain containing 1) genes on chromosome 17. The readthrough transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, May 2012]
Uniprot Description
AARSD1: Functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase
Chromosomal Location of Human Ortholog: 17q21.31
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; nucleic acid binding; metal ion binding; ATP binding; alanine-tRNA ligase activity
Biological Process: regulation of translational fidelity; alanyl-tRNA aminoacylation
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Product Notes
The AARSD1 aarsd1 (Catalog #AAA8107298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AARSD1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AARSD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the AARSD1 aarsd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AARSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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