Goat a2-Macroglobulin Polyclonal Antibody
a2-Macroglobulin
Reactivity
Canine, Human, Porcine, Rabbit
Applications
ELISA
Purity
PurifiedPurified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
Synonyms
a2-Macroglobulin; Polyclonal Antibody; Anti -a2-Macroglobulin; anti-a2-Macroglobulin antibody
Host
Goat
Reactivity
Canine, Human, Porcine, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Macroglobulin, alpha2.
Purity/Purification
Purified
Purified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
Purified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
Form/Format
Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.1M sodium chloride, 50% glycerol.
Applicable Applications for anti-a2-Macroglobulin antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA, Immunoprecipitation and Immunoelectrophoresis.
Immunogen
Human Macroglobulin, alpha2 purified from plasma.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-a2-Macroglobulin antibody
Alpha2 Macroglobulin (a-2M) is a large proteinase inhibitor molecule of 718kD, consisting of 4 identical subunits of 185,000 each. It is produced in hepatocytes and macrophages, and generally found in plasma concentrations of 2uM in adults, and up to 6uM in children. a-2M inhibits most enzymes from the serine, metallo, cysteine and aspartate subclasses. It is classed with proteins that include pregnancy zone protein (PZP) and the complement proteins C3, C4 and C5. These proteins contain regions of conserved sequence as well as one or more internal b-cysteinyl-g-glutamyl thiolester bonds. In a-2M, these regions are susceptible to cleavage by enzymes or by nucleophilic compounds such as methylamine or ammonium ions. It is thought that enzymatic cleavage of a bait region within the a-2M molecule causes a conformational change, which then traps and/or covalently binds the enzyme (1,2). The active site of the trapped enzyme is usually still intact and able to cleave small substrates, but is inaccessible to larger natural substrates. The conformational change also exposes receptor-binding regions within the molecule, which may be important in the clearance of a-2M enzyme complexes from the circulation. It is thought that the main role of a-2M in vivo is "backup" inhibitor and scavenger of proteinases in blood and in tissues (3,4). It has also been reported to participate in other physiological processes, including regulation of immune function (1,2).
Product Categories/Family for anti-a2-Macroglobulin antibody
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Product Notes
The a2-Macroglobulin (Catalog #AAA617996) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The a2-Macroglobulin reacts with Canine, Human, Porcine, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's a2-Macroglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA, Immunoprecipitation and Immunoelectrophoresis. Researchers should empirically determine the suitability of the a2-Macroglobulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a2-Macroglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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