Typical Testing Data/Standard Curve (for reference only)
Rat Brain mitochondrial carrier protein 1 (SLC25A14) ELISA Kit | SLC25A14 elisa kit
Rat Brain mitochondrial carrier protein 1 (SLC25A14) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SLC25A14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLC25A14 antibody and an SLC25A14-HRP conjugate. The assay sample and buffer are incubated together with SLC25A14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC25A14 concentration since SLC25A14 from samples and SLC25A14-HRP conjugate compete for the anti-SLC25A14 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC25A14 from the sample, fewer sites are left to bind SLC25A14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC25A14 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). Uncoupling proteins separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. Uncoupling proteins facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. This gene is widely expressed in many tissues with the greatest abundance in brain and testis. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 4. [provided by RefSeq, Aug 2013]
Uniprot Description
SLC25A14: Participates in the mitochondrial proton leak measured in brain mitochondria. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Mitochondrial
Chromosomal Location of Human Ortholog: Xq24
Cellular Component: mitochondrion; integral to plasma membrane; mitochondrial inner membrane
Biological Process: transport; aerobic respiration; mitochondrial transport
Research Articles on SLC25A14
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Product Notes
The Rat SLC25A14 slc25a14 (Catalog #AAA7235968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235968 ELISA Kit recognizes Rat SLC25A14. It is sometimes possible for the material contained within the vial of "Brain mitochondrial carrier protein 1 (SLC25A14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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