Western Blot (WB)
(Western blot analysis of 5T4 expression in Caco-2 cell lysates.)
Rabbit anti-Human 5T4 Polyclonal Antibody | anti-5T4 antibody
Anti-5T4 Rabbit pAb
Gene Names
TPBG; 5T4; M6P1; 5T4AG; WAIF1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
5T4; Polyclonal Antibody; Anti-5T4 Rabbit pAb; TPBG; 5T4-AG; 5T4AG; 5T4 oncofetal antigen; M6P1; Trophoblast glycoprotein; anti-5T4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
5T4 Antibody detects endogenous levels of 5T4
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
420
Applicable Applications for anti-5T4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human 5T4
Subcellular Location
Chromosome
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of 5T4 expression in Caco-2 cell lysates.)
Western Blot (WB)
(Western blot analysis of 5T4 expression in Caco-2 cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uters cancer, using 5T4 Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uters cancer, using 5T4 Antibody.)
Related Product Information for anti-5T4 antibody
5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1-60.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80kDa
NCBI Official Full Name
trophoblast glycoprotein
NCBI Official Synonym Full Names
trophoblast glycoprotein
NCBI Official Symbol
TPBG
NCBI Official Synonym Symbols
5T4; M6P1; 5T4AG; WAIF1
NCBI Protein Information
trophoblast glycoprotein
UniProt Protein Name
Trophoblast glycoprotein
UniProt Gene Name
TPBG
UniProt Synonym Gene Names
5T4; 5T4 oncotrophoblast glycoprotein; WAIF1
NCBI Description
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]
Uniprot Description
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Research Articles on 5T4
Similar Products
Product Notes
The 5T4 tpbg (Catalog #AAA4750103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-5T4 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 5T4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the 5T4 tpbg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5T4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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