Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Human ABAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human 4-Aminobutyrate Aminotransferase Polyclonal Antibody | anti-ABAT antibody
Polyclonal Antibody to 4-Aminobutyrate Aminotransferase (ABAT)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Human ABAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Human ABAT Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
NCBI and Uniprot Product Information
NCBI Description
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ABAT: Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. Defects in ABAT are a cause of GABA transaminase deficiency (GABATD). The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; EC 2.6.1.19; EC 2.6.1.22; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Transferase
Chromosomal Location of Human Ortholog: 16p13.2
Cellular Component: 4-aminobutyrate transaminase complex; mitochondrial matrix; mitochondrion; neuron projection
Molecular Function: (S)-3-amino-2-methylpropionate transaminase activity; 4-aminobutyrate transaminase activity; iron-sulfur cluster binding; metal ion binding; protein homodimerization activity; pyridoxal phosphate binding; succinate-semialdehyde dehydrogenase binding
Biological Process: aging; behavioral response to cocaine; cerebellum development; copulation; gamma-aminobutyric acid biosynthetic process; gamma-aminobutyric acid catabolic process; locomotory behavior; negative regulation of blood pressure; negative regulation of dopamine secretion; negative regulation of gamma-aminobutyric acid secretion; neurotransmitter catabolic process; positive regulation of dopamine metabolic process; positive regulation of heat generation; positive regulation of insulin secretion; response to drug; response to ethanol; response to hypoxia; response to iron ion; response to nicotine
Disease: Gaba-transaminase Deficiency
Research Articles on ABAT
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Product Notes
The ABAT abat (Catalog #AAA2033835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to 4-Aminobutyrate Aminotransferase (ABAT) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 4-Aminobutyrate Aminotransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the ABAT abat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-Aminobutyrate Aminotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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