Rabbit 2',5'-Oligoadenylate Synthetase 1 (OAS1) Polyclonal Antibody | anti-OAS1 antibody

Polyclonal Antibody to 2',5'-Oligoadenylate Synthetase 1 (OAS1)

Cat. Num. AAA2004137

• Gene Names: OAS1; OIAS; IFI-4; OIASI; E18/E16
• Reactivity: Homo sapiens (Human)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: 2'; 5'-Oligoadenylate Synthetase 1 (OAS1); Polyclonal Antibody; Polyclonal Antibody to 2'; anti-OAS1 antibody

• Host: Rabbit
• Reactivity: Homo sapiens (Human)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.24mg/ml (varies by lot)

• Applicable Applications for anti-OAS1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-5ug/mL; Immunohistochemistry: 5-50ug/mL; Immunocytochemistry: 5-50ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Pig
• Immunogen: Recombinant OAS1 (Ser74~Pro165) expressed in E.coli ( MBS2011404 ).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Sample: Recombinant OAS1, Human.)

Western Blot (WB)

(Western blot; Sample: Porcine Cerebrum lysate;Primary Ab: 5ug/ml Rabbit Anti-Human OAS1 antibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Colorectal cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-HumanOAS1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded stomach cancer tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 74229015
• NCBI GeneID: 4938
• NCBI Accession #: NP_001027581.1
• NCBI GenBank Nucleotide #: NM_001032409.2
• UniProt Accession #: P00973; P04820; P29080; P29081; P78485; P78486; Q16700; Q16701; Q1PG42; Q3ZM01; Q53GC5; A8K4N8
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 1 isoform 3
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 1
• NCBI Official Symbol: OAS1
• NCBI Official Synonym Symbols: OIAS; IFI-4; OIASI; E18/E16
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 1
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 1
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS1
• UniProt Synonym Gene Names: OIAS; (2-5')oligo(A) synthase 1; 2-5A synthase 1

NCBI Description

This gene is induced by interferons and encodes a protein that synthesizes 2',5'-oligoadenylates (2-5As). This protein activates latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. Alternative splicing results in multiple transcript variants with different enzymatic activities. Polymorphisms in this gene have been associated with susceptibility to viral infection and diabetes mellitus, type 1. A disease-associated allele in a splice acceptor site influences the production of the p46 splice isoform. This gene is located in a cluster of related genes on chromosome 12. [provided by RefSeq, Feb 2016]

Uniprot Description

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.