Rabbit 14-3-3, tau Polyclonal Antibody | anti-YWHAQ antibody

14-3-3, tau (14-3-3 Protein theta, YWHAQ, 1C5, HS1, 14-3-3) (Biotin)

Cat. Num. AAA6404894

• Gene Names: YWHAQ; 1C5; HS1; 14-3-3
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: 14-3-3; tau; Polyclonal Antibody; tau (14-3-3 Protein theta; YWHAQ; 1C5; HS1; 14-3-3) (Biotin); anti-YWHAQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes YWHAQ. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-YWHAQ antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:100; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human YWHAQ.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using YWHAQ antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart using YWHAQ)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using YWHAQ. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-YWHAQ antibody

Antibodies; 14-3-3

NCBI and Uniprot Product Information

• NCBI GI #: 5803227
• NCBI GeneID: 10971
• NCBI Accession #: NP_006817.1
• NCBI GenBank Nucleotide #: NM_006826.3
• UniProt Accession #: P27348; Q567U5; Q5TZU8; Q9UP48; D6W4Z5
• Molecular Weight: 27,764 Da
• NCBI Official Full Name: 14-3-3 protein theta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• NCBI Official Symbol: YWHAQ
• NCBI Official Synonym Symbols: 1C5; HS1; 14-3-3
• NCBI Protein Information: 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell
• UniProt Protein Name: 14-3-3 protein theta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAQ
• UniProt Entry Name: 1433T_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting