Western Blot (WB)
(All lanes: 14-3-3 protein beta/alpha antibody at 2ug/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29,28 kDaObserved band size: 27 kDa)
Rabbit 14-3-3 protein beta/alpha Polyclonal Antibody | anti-YWHAB antibody
Rabbit anti-human 14-3-3 protein beta/alpha polyclonal Antibody
Western Blot (WB)
(All lanes: 14-3-3 protein beta/alpha antibody at 2ug/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29,28 kDaObserved band size: 27 kDa)
Western Blot (WB)
(All lanes: 14-3-3 protein beta/alpha antibody at 2ug/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 29,28 kDaObserved band size: 27 kDa)
Western Blot (WB)
(Western blotAll lanes: YWHAB antibody at 6ug/mlLane 1: Hela whole cell lysateLane 2: Raji whole cell lysateLane 3: Mouse liver tissueLane 4: Mouse brain tissueLane 5: Rat liver tissueLane 6: Rat lung tissueLane 7: Mouse lung tissueLane 8: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 29,28 kDaObserved band size: 28,39,64 kDa)
Western Blot (WB)
(Western blotAll lanes: YWHAB antibody at 6ug/mlLane 1: Hela whole cell lysateLane 2: Raji whole cell lysateLane 3: Mouse liver tissueLane 4: Mouse brain tissueLane 5: Rat liver tissueLane 6: Rat lung tissueLane 7: Mouse lung tissueLane 8: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 29,28 kDaObserved band size: 28,39,64 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysateAll lanes: YWHAB antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,28 KDaObserved band size: 29 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysateAll lanes: YWHAB antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,28 KDaObserved band size: 29 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7002751 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7002751 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7002751 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7002751 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7002751 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7002751 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 20q13.1
Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; melanosome; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; transcriptional repressor complex
Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; protein binding; protein C-terminus binding; protein complex binding; protein domain specific binding; transcription corepressor activity
Biological Process: activation of MAPKK activity; apoptosis; axon guidance; cytoplasmic sequestering of protein; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of protein amino acid dephosphorylation; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; positive regulation of catalytic activity; programmed cell death; protein heterooligomerization; protein targeting; Ras protein signal transduction; regulation of mRNA stability; small GTPase mediated signal transduction; transcription initiation from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on YWHAB
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Product Notes
The YWHAB ywhab (Catalog #AAA7002751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human 14-3-3 protein beta/alpha polyclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 protein beta/alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the YWHAB ywhab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 protein beta/alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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