Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 g antibody.)
Rabbit 14-3-3 gamma Polyclonal Antibody | anti-14-3-3 gamma antibody
14-3-3 gamma Antibody
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 g antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 g antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 g antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 g antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using 14-3-3 g antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using 14-3-3 g antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
Uniprot Description
14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol
Molecular Function: protein kinase C inhibitor activity; protein domain specific binding; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding
Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; negative regulation of protein kinase activity; regulation of synaptic plasticity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting
Research Articles on 14-3-3 gamma
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Product Notes
The 14-3-3 gamma ywhag (Catalog #AAA9409748) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3 gamma Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the 14-3-3 gamma ywhag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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