POLR3A recombinant protein

Recombinant Human POLR3A protein, N- His Tag & sumo Tag

Cat. Num. AAA157529

• Gene Names: POLR3A; ADDH; HLD7; RPC1; RPC155; hRPC155
• Purity: >90% as determined by SDS-PAGE
• Synonyms: POLR3A; Recombinant Human POLR3A protein; N- His Tag & sumo Tag; RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160; POLR3A recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
• Construction: A DNA sequence encoding the Human POLR3A (Phe392-Cys632) was fused with the N-terminal His Tag & sumo Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise

Product Categories/Family for POLR3A recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 39725938
• NCBI GeneID: 11128
• NCBI Accession #: NP_008986.2
• NCBI GenBank Nucleotide #: NM_007055.3
• UniProt Accession #: O14802; Q8IW34; Q8TCW5
• Molecular Weight: 155,641 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC1
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• NCBI Official Symbol: POLR3A
• NCBI Official Synonym Symbols: ADDH; HLD7; RPC1; RPC155; hRPC155
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; RNA polymerase III subunit C160; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit RPC155-D; DNA-directed RNA polymerase III subunit A; DNA-directed RNA polymerase I
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC1
• UniProt Gene Name: POLR3A
• UniProt Synonym Gene Names: RNA polymerase III subunit C1; RPC155
• UniProt Entry Name: RPC1_HUMAN

NCBI Description

The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]

Uniprot Description

POLR3A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3A are the cause of leukodystrophy hypomyelinating type 7 with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7). An autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability. Belongs to the RNA polymerase beta' chain family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex; Transferase; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; membrane; cytosol

Molecular Function: DNA binding; zinc ion binding; ribonucleoside binding; DNA-directed RNA polymerase activity; chromatin binding

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism