DNA-directed RNA polymerase III subunit RPC1 (POLR3A) Recombinant Protein | POLR3A recombinant protein

Recombinant Bovine DNA-directed RNA polymerase III subunit RPC1 (POLR3A) , partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase III subunit RPC1 (POLR3A); Recombinant Bovine DNA-directed RNA polymerase III subunit RPC1 (POLR3A); partial; POLR3A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,390

• Species: Bovine
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 139948582
• NCBI GeneID: 540308
• NCBI Accession #: NP_001077219.1
• NCBI GenBank Nucleotide #: NM_001083750.1
• UniProt Accession #: A4IF62
• Molecular Weight: 155,791 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC1
• NCBI Official Synonym Full Names: RNA polymerase III subunit A
• NCBI Official Symbol: POLR3A
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC1
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC1
• UniProt Gene Name: POLR3A
• UniProt Synonym Gene Names: RNA polymerase III subunit C1

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway ().