Polymerase II Recombinant Protein | POLR2J recombinant protein

Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J

Cat. Num. AAA145817

• Gene Names: POLR2J; RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Polymerase II; Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J; POLR2J Human; Polymerase (RNA) II (DNA directed) Polypeptide J Human Recombinant; DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit B11-a; RPB11a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit; POLR2J; POLR2J1; RPB11; RPB11m; hRPB14; POLR2J recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: POLR2J protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE.
• Sequence Length: 117

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for POLR2J recombinant protein

Description: POLR2J Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117 a.a) and having a molecular mass of 15.7kDa.POLR2J is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Polymerase (RNA) II (DNA directed) Polypeptide J (POLR2J) is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The POLR2J protein occurs as a heterodimer with another polymerase subunit; together they develop a core subassembly unit of the polymerase. POLR2J is ubiquitously expressed, having a high expression in the heart and skeletal muscle.

Product Categories/Family for POLR2J recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Polymerase (RNA) (DNA directed) Polypeptide

NCBI and Uniprot Product Information

• NCBI GI #: 5453932
• NCBI GeneID: 5439
• NCBI Accession #: NP_006225.1
• NCBI GenBank Nucleotide #: NM_006234.4
• UniProt Accession #: P52435; O43375; A5D6V8
• Molecular Weight: 13,293 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB11-a
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
• NCBI Official Symbol: POLR2J
• NCBI Official Synonym Symbols: RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB11-a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit; RNA polymerase II subunit B11-a
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB11-a
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2J
• UniProt Synonym Gene Names: POLR2J1; RNA polymerase II subunit B11-a; RPB11a
• UniProt Entry Name: RPB11_HUMAN

NCBI Description

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Protein type: RNA processing; RNA splicing; DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; DNA-directed RNA polymerase activity; LRR domain binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; nucleotide-excision repair; somatic stem cell maintenance; positive regulation of viral transcription; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair