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SDS-Page

POLR2F recombinant protein

POLR2F, 1-127aa, Human, His tag, E Coli

Gene Names
POLR2F; RPB6; POLRF; RPC15; RPABC2; RPB14.4; HRBP14.4; RPABC14.4
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
POLR2F; 1-127aa; Human; His tag; E Coli; DNA-directed RNA polymerases I II and III subunit RPABC2; DNA-directed RNA polymerases I; II; and III subunit RPABC2; Polymerase (RNA) II (DNA directed) polypeptide F; HRBP14.4; POLRF; RPABC2; RPB14.4; RPB6; POLR2F recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD
Sequence Length
127
Applicable Applications for POLR2F recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for POLR2F recombinant protein
POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for POLR2F recombinant protein
References
Kayukawa K., Makino Y., et al. (1999) Gene 234:139-147

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16.9kDa (150aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC2 isoform 1
NCBI Official Synonym Full Names
RNA polymerase II subunit F
NCBI Official Symbol
POLR2F
NCBI Official Synonym Symbols
RPB6; POLRF; RPC15; RPABC2; RPB14.4; HRBP14.4; RPABC14.4
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC2
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC2
UniProt Gene Name
POLR2F
UniProt Synonym Gene Names
POLRF; RNA polymerases I, II, and III subunit ABC2; RPB14.4

NCBI Description

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds ().

Research Articles on POLR2F

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Product Notes

The POLR2F polr2f (Catalog #AAA205739) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's POLR2F can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the POLR2F polr2f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD. It is sometimes possible for the material contained within the vial of "POLR2F, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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