DNA-directed RNA polymerase II subunit RPB2 (POLR2B) Recombinant Protein | POLR2B recombinant protein

Recombinant Human DNA-directed RNA polymerase II subunit RPB2 (POLR2B) , partial

• Gene Names: POLR2B; RPB2; POL2RB; hRPB140
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase II subunit RPB2 (POLR2B); Recombinant Human DNA-directed RNA polymerase II subunit RPB2 (POLR2B); partial; POLR2B recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,174

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for POLR2B recombinant protein

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 4505941
• NCBI GeneID: 5431
• NCBI Accession #: NP_000929.1
• NCBI GenBank Nucleotide #: NM_000938.2
• UniProt Accession #: P30876; Q8IZ61; A8K1A8
• Molecular Weight: 133,897 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB2 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase II subunit B
• NCBI Official Symbol: POLR2B
• NCBI Official Synonym Symbols: RPB2; POL2RB; hRPB140
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB2
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2B

NCBI Description

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template ().