Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa ELISA Kit | POLR2B elisa kit

Human DNA-directed RNA polymerase II subunit RPB2, POLR2B ELISA Kit

Cat. Num. AAA9332767

• Gene Names: POLR2B; RPB2; POL2RB; hsRPB2; hRPB140
• Reactivity: Human
• Synonyms: polymerase (RNA) II (DNA directed) polypeptide B; 140kDa; Human DNA-directed RNA polymerase II subunit RPB2; POLR2B ELISA Kit; Human DNA-directed RNA polymerase II subunit RPB2 (POLR2B) ELISA kit; POL2RB; RPB2; hRPB140; hsRPB2; DNA directed RNA polymerase II 140 kDa polypeptide; RNA polymerase II subunit 2; RNA polymerase II second largest subunit; DNA directed RNA polymerase II polypeptide B; ; POLR2B elisa kit

• Reactivity: Human

• Samples: Undiluted original Human serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for POLR2B elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of POLR2B (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 4505941
• NCBI GeneID: 5431
• NCBI Accession #: NP_000929.1
• NCBI GenBank Nucleotide #: NM_000938.1
• UniProt Accession #: P30876; Q8IZ61; A8K1A8; AAB23139
• Molecular Weight: 133,897 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB2
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
• NCBI Official Symbol: POLR2B
• NCBI Official Synonym Symbols: RPB2; POL2RB; hsRPB2; hRPB140
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB2; RNA polymerase II subunit 2; RNA polymerase II subunit B2; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA directed RNA polymerase II 140 kDa polypeptide, RNA polymerase II subunit 2, RNA polymerase II second largest subunit
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2B
• UniProt Entry Name: RPB2_HUMAN

NCBI Description

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2B: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. Belongs to the RNA polymerase beta chain family.

Protein type: RNA splicing; RNA-binding; Nucleotide Metabolism - purine; EC 2.7.7.6; Transcription initiation complex; RNA processing; Transferase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; membrane; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein binding; DNA binding; ribonucleoside binding; metal ion binding; chromatin binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair