Related Product Information for PNPLA2 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PNPLA2 in samples. An antibody specific for PNPLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Uniprot Description
PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.1.3; Lipase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: cytoplasm; cytosol; integral component of membrane; lipid droplet; membrane; nucleoplasm; plasma membrane
Molecular Function: protein binding; triacylglycerol lipase activity
Biological Process: cellular lipid catabolic process; lipid homeostasis; lipid storage; triglyceride catabolic process
Product Notes
The Rat PNPLA2 pnpla2 (Catalog #
AAA285410) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285410 ELISA Kit recognizes Rat PNPLA2.
It is sometimes possible for the material contained within the vial of
"Adipose Triglyceride Lipase, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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