Human Phorbol-12-myristate-13-acetate-induced protein 1 ELISA Kit | PMAIP1 elisa kit

Human Phorbol-12-myristate-13-acetate-induced protein 1 ELISA Kit

Cat. Num. AAA761963

• Gene Names: PMAIP1; APR; NOXA
• Reactivity: Human
• Synonyms: Phorbol-12-myristate-13-acetate-induced protein 1; Human Phorbol-12-myristate-13-acetate-induced protein 1 ELISA Kit; PMAIP1/PMA-induced protein 1/Phorbol-12-myristate-13-acetate-induced protein 1/NOXA/Immediate-early-response protein APR/Protein Noxa; PMAIP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PMAIP1. No significant cross-reactivity or interference between PMAIP1 and analogues was observed.
• Sequence Length: 54

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/ml
• Sensitivity: <46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PMAIP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PMAIP1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PMAIP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PMAIP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PMAIP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PMAIP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PMAIP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10863923
• NCBI GeneID: 5366
• NCBI Accession #: NP_066950.1
• NCBI GenBank Nucleotide #: NM_021127.2
• Molecular Weight: 14,955 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: PMAIP1
• NCBI Official Synonym Symbols: APR; NOXA
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Phorbol-12-myristate-13-acetate-induced protein
• UniProt Gene Name: PMAIP1
• UniProt Synonym Gene Names: NOXA; PMA-induced protein 1
• UniProt Entry Name: APR_HUMAN

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion; nucleus

Molecular Function: protein binding

Biological Process: apoptosis; caspase activation; cellular response to glucose starvation; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of fibroblast proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of neuron apoptosis; positive regulation of protein oligomerization; programmed cell death; proteasomal protein catabolic process; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; response to dsRNA; response to UV; response to X-ray; T cell homeostasis