Chicken Phospholipid Scramblase 2 ELISA Kit | PLSCR2 elisa kit

Chicken Phospholipid Scramblase 2 ELISA Kit

Cat. Num. AAA743512

• Reactivity: Chicken
• Synonyms: Phospholipid Scramblase 2; Chicken Phospholipid Scramblase 2 ELISA Kit; PLSCR2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLSCR2 elisa kit

Intended Uses: This PLSCR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PLSCR2. This ELISA kit for research use only!

Principle of the Assay: PLSCR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLSCR2 antibody and an PLSCR2-HRP conjugate. The assay sample and buffer are incubated together with PLSCR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLSCR2 concentration since PLSCR2 from samples and PLSCR2-HRP conjugate compete for the anti-PLSCR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLSCR2 from the sample, fewer sites are left to bind PLSCR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLSCR2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLSCR2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 317171904
• NCBI GeneID: 57047
• NCBI Accession #: NP_001186907.1
• NCBI GenBank Nucleotide #: NM_001199978.1
• UniProt Accession #: Q7Z4L7; Q9NRY7
• Molecular Weight: 35,766 Da
• NCBI Official Full Name: phospholipid scramblase 2 isoform 1
• NCBI Official Synonym Full Names: phospholipid scramblase 2
• NCBI Official Symbol: PLSCR2
• NCBI Protein Information: phospholipid scramblase 2; PL scramblase 2; ca(2+)-dependent phospholipid scramblase 2
• UniProt Protein Name: PLSCR2 protein
• Protein Family: Phospholipid scramblase
• UniProt Gene Name: PLSCR2
• UniProt Entry Name: Q7Z4L7_HUMAN

NCBI Description

This gene encodes a member of the phospholipid scramblase family. Phospholipid scramblases are membrane proteins that mediate calcium-dependent, non-specific movement of plasma membrane phospholipids and phosphatidylserine exposure. The encoded protein contains a low affinity calcium binding motif and may play a role in blood coagulation and apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PLSCR2: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family.

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral to membrane; plasma membrane; nucleus

Molecular Function: phospholipid scramblase activity; calcium ion binding

Biological Process: phospholipid scrambling