Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Diacylglycerol Kinase Gamma (DGKg) Polyclonal Antibody | anti-DGKg antibody
Polyclonal Antibody to Diacylglycerol Kinase Gamma (DGKg)
Gene Names
Dgkg; Dagk3; DGKIII
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Diacylglycerol Kinase Gamma (DGKg); Polyclonal Antibody; Polyclonal Antibody to Diacylglycerol Kinase Gamma (DGKg); anti-DGKg antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against DGKg. It has been selected for its ability to recognize DGKg in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- IMLM GVRKQGLCCI YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR DRQAGKSDSG AAAKGELVMQ YKIIPSPGTH PLLVLVNPKS GGRQGERILQ KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- IMLM GVRKQGLCCI YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR DRQAGKSDSG AAAKGELVMQ YKIIPSPGTH PLLVLVNPKS GGRQGERILQ KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP
Sequence Length
791
Applicable Applications for anti-DGKg antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant DGKg (Ile287~Arg524) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075661
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,521 Da
NCBI Official Full Name
diacylglycerol kinase gamma
NCBI Official Synonym Full Names
diacylglycerol kinase, gamma
NCBI Official Symbol
Dgkg
NCBI Official Synonym Symbols
Dagk3; DGKIII
NCBI Protein Information
diacylglycerol kinase gamma
UniProt Protein Name
Diacylglycerol kinase gamma
Protein Family
UniProt Gene Name
Dgkg
UniProt Synonym Gene Names
Dagk3; DAG kinase gamma; DGK-gamma
NCBI Description
putative diacylglycerol kinase associated with the cytoskeleton of purkinje cells [RGD, Feb 2006]
Uniprot Description
DGKG: Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: intracellular
Molecular Function: ATP binding; calcium ion binding; diacylglycerol kinase activity; NAD+ kinase activity
Biological Process: glycerolipid metabolic process; lipid phosphorylation; neuron development; protein kinase C-activating G-protein coupled receptor signaling pathway
Research Articles on DGKg
Similar Products
Product Notes
The DGKg dgkg (Catalog #AAA2013107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Diacylglycerol Kinase Gamma (DGKg) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Diacylglycerol Kinase Gamma (DGKg) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the DGKg dgkg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- IMLM GVRKQGLCCI YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR DRQAGKSDSG AAAKGELVMQ YKIIPSPGTH PLLVLVNPKS GGRQGERILQ KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP. It is sometimes possible for the material contained within the vial of "Diacylglycerol Kinase Gamma (DGKg), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
