Human Plasminogen Activator ELISA Kit | PLGA elisa kit

Human Plasminogen Activator ELISA Kit

Cat. Num. AAA763376

• Gene Names: PLAT; TPA; T-PA
• Reactivity: Human
• Synonyms: Plasminogen Activator; Human Plasminogen Activator ELISA Kit; PLGA; PLGA elisa kit

• Reactivity: Human
• Sequence Length: 562

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PLGA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PLGA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PLGA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PLGA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PLGA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 339834
• NCBI GeneID: 5327
• Molecular Weight: 44,373 Da
• NCBI Official Full Name: plasminogen activator
• NCBI Official Synonym Full Names: plasminogen activator, tissue type
• NCBI Official Symbol: PLAT
• NCBI Official Synonym Symbols: TPA; T-PA
• NCBI Protein Information: tissue-type plasminogen activator
• UniProt Protein Name: Tissue-type plasminogen activator
• Protein Family: Plasminogen activator inhibitor
• UniProt Gene Name: PLAT
• UniProt Synonym Gene Names: t-PA; t-plasminogen activator; tPA
• UniProt Entry Name: TPA_HUMAN

NCBI Description

This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; EC 3.4.21.68; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular region

Molecular Function: glycoprotein binding; phosphoprotein binding; protein binding; receptor binding; serine-type endopeptidase activity

Biological Process: blood coagulation; fibrinolysis; negative regulation of proteolysis; plasminogen activation; protein modification process; proteolysis

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator