Human Pleckstrin ELISA Kit | PLEK elisa kit
Human Pleckstrin (PLEK) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PDGFD in Human serum, plasma. An antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
pleckstrin: Major protein kinase C substrate of platelets.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: cytoplasm; cytosol; extracellular region; membrane
Molecular Function: phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase C binding
Biological Process: actin cytoskeleton reorganization; cell projection organization; cortical actin cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; integrin-mediated signaling pathway; negative regulation of calcium-mediated signaling; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of inositol phosphate biosynthetic process; phosphatidylinositol metabolic process; phospholipase C inhibition; platelet degranulation; positive regulation of actin filament bundle assembly; positive regulation of actin filament depolymerization; positive regulation of inositol-polyphosphate 5-phosphatase activity; positive regulation of integrin activation; positive regulation of platelet activation; ruffle organization; vesicle docking involved in exocytosis
Research Articles on PLEK
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Product Notes
The Human PLEK plek (Catalog #AAA281194) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281194 ELISA Kit recognizes Human PLEK. It is sometimes possible for the material contained within the vial of "Pleckstrin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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