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Mouse Urokinase-type Plasminogen Activator Receptor ELISA Kit | PLAUR/uPAR elisa kit

Mouse Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit

Reactivity
Mouse
Synonyms
Urokinase-type Plasminogen Activator Receptor; Mouse Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit; CD87; UPAR; URKR; monocyte activation antigen Mo3|u-plasminogen activator receptor form 2; PLAUR/uPAR elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse PLAUR/uPAR. No significant cross-reactivity or interference between Mouse PLAUR/uPAR and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PLAUR/uPAR elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLAUR/uPAR in samples. An antibody specific for PLAUR/uPAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAUR/uPAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAUR/uPAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAUR/uPAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,619 Da
NCBI Official Full Name
urokinase plasminogen activator surface receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
Plaur
UniProt Synonym Gene Names
U-PAR; uPAR

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 7|7 A3

Cellular Component: cell surface; extracellular region; focal adhesion; integral component of plasma membrane; membrane; plasma membrane

Molecular Function: enzyme binding; protein binding; protein domain specific binding; receptor binding; U-plasminogen activator receptor activity

Biological Process: mesenchymal cell differentiation; negative regulation of apoptosis; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of protein phosphorylation; regulation of proteolysis

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Product Notes

The Mouse PLAUR/uPAR plaur (Catalog #AAA284254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284254 ELISA Kit recognizes Mouse PLAUR/uPAR. It is sometimes possible for the material contained within the vial of "Urokinase-type Plasminogen Activator Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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