Typical Testing Data/Standard Curve (for reference only)
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Canine Urokinase-type Plasminogen Activator Receptor ELISA Kit | PLAUR/Upar elisa kit
Canine Urokinase-type Plasminogen Activator Receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: uPAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-uPAR antibody and an uPAR-HRP conjugate. The assay sample and buffer are incubated together with uPAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the uPAR concentration since uPAR from samples and uPAR-HRP conjugate compete for the anti-uPAR antibody binding site. Since the number of sites is limited, as more sites are occupied by uPAR from the sample, fewer sites are left to bind uPAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The uPAR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]
Uniprot Description
uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: endoplasmic reticulum membrane; focal adhesion; extrinsic to membrane; integral to plasma membrane; endoplasmic reticulum lumen; plasma membrane; integral to membrane
Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor activity; receptor binding
Biological Process: fibrinolysis; cellular protein metabolic process; regulation of proteolysis; attachment of GPI anchor to protein; C-terminal protein lipidation; post-translational protein modification; signal transduction; chemotaxis; cell motility; blood coagulation
Research Articles on PLAUR/Upar
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Product Notes
The Canine PLAUR/Upar plaur (Catalog #AAA737026) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737026 ELISA Kit recognizes Canine PLAUR/Upar. It is sometimes possible for the material contained within the vial of "Urokinase-type Plasminogen Activator Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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