Goat Urokinase-type Plasminogen Activator Receptor ELISA Kit | u-PAR elisa kit

Goat Urokinase-type Plasminogen Activator Receptor ELISA Kit

Cat. Num. AAA737328

• Gene Names: PLAUR; CD87; UPAR; URKR; U-PAR
• Reactivity: Goat
• Synonyms: Urokinase-type Plasminogen Activator Receptor; Goat Urokinase-type Plasminogen Activator Receptor ELISA Kit; u-PAR elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This u-PAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat u-PAR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for u-PAR elisa kit

Principle of the Assay: u-PAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-u-PAR antibody and an u-PAR-HRP conjugate. The assay sample and buffer are incubated together with u-PAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the u-PAR concentration since u-PAR from samples and u-PAR-HRP conjugate compete for the anti-u-PAR antibody binding site. Since the number of sites is limited, as more sites are occupied by u-PAR from the sample, fewer sites are left to bind u-PAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The u-PAR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for u-PAR elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 517198
• NCBI GeneID: 5329
• UniProt Accession #: Q03405; Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409; Q6GY32; Q9UPI5
• Molecular Weight: 36,978 Da
• NCBI Official Full Name: urokinase-type plasminogen activator receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: PLAUR
• NCBI Official Synonym Symbols: CD87; UPAR; URKR; U-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR; U-PAR; uPAR
• UniProt Entry Name: UPAR_HUMAN

NCBI Description

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; endoplasmic reticulum lumen; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein domain specific binding; protein binding; enzyme binding; U-plasminogen activator receptor activity; receptor activity; receptor binding

Biological Process: fibrinolysis; cellular protein metabolic process; attachment of GPI anchor to protein; regulation of proteolysis; C-terminal protein lipidation; chemotaxis; cell motility; signal transduction; blood coagulation; post-translational protein modification