Sequence Information
Plasminogen Activator Recombinant Protein | tPA recombinant protein
Recombinant Plasminogen Activator, Tissue (tPA)
Source: Prokaryotic expression
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GGLFTDITSH PWQAAIFVKN KRSPGERFLC GGVLISSCWV LSAAHCFVER FPPHHLKVVL GRTYRVVPGE EEQTFEIEKY IVHKEFDDDT YDNDIALLQL RSDSSQCAQE SSSVGTACLP DPDVQLPDWT ECELSGYGKHEASSPFFSDR LKEAHVRLYP SSRCTSQHLF NKTITSN
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Protease; Cytoskeletal; Secreted, signal peptide; EC 3.4.21.68; Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule
Molecular Function: protein binding; serine-type endopeptidase activity
Biological Process: negative regulation of proteolysis; response to peptide hormone stimulus; synaptic transmission, glutamatergic; response to cAMP; smooth muscle cell migration; protein modification process; regulation of synaptic plasticity; platelet-derived growth factor receptor signaling pathway; proteolysis; fibrinolysis; response to hypoxia; blood coagulation; transmembrane transport; plasminogen activation
Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
Research Articles on tPA
Similar Products
Product Notes
The tPA plat (Catalog #AAA2030532) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Plasminogen Activator can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the tPA plat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- GGLFTDITSH PWQAAIFVKN KRSPGERFLC GGVLISSCWV LSAAHCFVER FPPHHLKVVL GRTYRVVPGE EEQTFEIEKY IVHKEFDDDT YDNDIALLQL RSDSSQCAQE SSSVGTACLP DPDVQLPDWT ECELSGYGKH EASSPFFSDR LKEAHVRLYP SSRCTSQHLF NKTITSN. It is sometimes possible for the material contained within the vial of "Plasminogen Activator, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
