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PLA2G1B recombinant protein
PLA2G1B Protein, Mouse, Recombinant (His Tag)
Gene Names
Pla2g1b; Pla2a; sPLA2IB
Purity
>96% as determined by SDS-PAGE
Synonyms
PLA2G1B; PLA2G1B Protein; Mouse; Recombinant (His Tag); Mouse PLA2G1B/Phospholipase-A2 Protein (His Tag); Pla2a Protein; sPLA2IB Protein; phospholipase A2; group IB (pancreas); PLA2G1B recombinant protein
Host
HEK293 Cells
Purity/Purification
>96% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Cys146
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Ala 16
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse PLA2G1B (NP_035237.1) extracellular domain (Met 1-Cys 146) was fused with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for PLA2G1B recombinant protein
Background: phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2/PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2/PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2/PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2/PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.
References
Labonta©,E.D. et al., 2006, Diabetes. 55 (4) :935-41. Mounier,C.M. et al.,2008, Br J Cancer. 98 (3):587-95. Hui,D.Y. et al., 2009, Br J Pharmacol. 157 (7):1263-9. Labonta©,E.D. et al., 2010, FASEB J. 24 (7):2516-24.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,290 Da
NCBI Official Full Name
phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IB, pancreas
NCBI Official Symbol
Pla2g1b
NCBI Official Synonym Symbols
Pla2a; sPLA2IB
NCBI Protein Information
phospholipase A2
UniProt Protein Name
Phospholipase A2
Protein Family
UniProt Gene Name
Pla2g1b
UniProt Synonym Gene Names
Pla2
UniProt Entry Name
PA21B_MOUSE
Uniprot Description
PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.
Protein type: DNA replication; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Phospholipase; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Secreted; Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - ether lipid
Cellular Component: cell surface; extracellular region; extracellular space; secretory granule
Molecular Function: calcium ion binding; calcium-dependent phospholipase A2 activity; hydrolase activity; metal ion binding; phospholipase A2 activity; receptor binding
Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; fatty acid biosynthetic process; innate immune response in mucosa; lipid catabolic process; lipid metabolic process; multicellular organismal lipid catabolic process; phosphatidylcholine metabolic process; phospholipid catabolic process; phospholipid metabolic process; positive regulation of DNA replication
Research Articles on PLA2G1B
Similar Products
Product Notes
The PLA2G1B pla2g1b (Catalog #AAA8122689) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Cys14 6. It is sometimes possible for the material contained within the vial of "PLA2G1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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