PLA1A recombinant protein

PLA1A, 26-456aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205619

• Gene Names: PLA1A; PSPLA1; PS-PLA1
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: PLA1A; 26-456aa; Human; His tag; E Coli (Denatured); Phospholipase A1 member A; PS-PLA1; PSPLA1; PLA1A recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV
• Sequence Length: 456

• Applicable Applications for PLA1A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PLA1A recombinant protein

PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human PLA1A protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for PLA1A recombinant protein

Cardiovascular

References

Wang J., et al. (2002) J. Hum. Genet. 47:611-613; Nagai Y., et al. (1999) J. Biol. Chem. 274:11053-11059;

NCBI and Uniprot Product Information

• NCBI GI #: 7706661
• NCBI GeneID: 51365
• NCBI Accession #: NP_056984
• NCBI GenBank Nucleotide #: NM_015900.3
• UniProt Accession #: Q53H76; O95991; Q86WX6; Q9UPD2; B2R8V2; B4DXA2
• Molecular Weight: 49.5 kDa (454aa)
• NCBI Official Full Name: phospholipase A1 member A isoform 1
• NCBI Official Synonym Full Names: phospholipase A1 member A
• NCBI Official Symbol: PLA1A
• NCBI Official Synonym Symbols: PSPLA1; PS-PLA1
• NCBI Protein Information: phospholipase A1 member A
• UniProt Protein Name: Phospholipase A1 member A
• Protein Family: Phospholipase
• UniProt Gene Name: PLA1A
• UniProt Synonym Gene Names: NMD; PSPLA1; PS-PLA1

NCBI Description

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.