Rabbit anti-Human WISP3 Polyclonal Antibody | anti-WISP3 antibody

WISP3, ID (WNT1-inducible-signaling Pathway Protein 3, WISP-3, CCN Family Member 6, CCN6, UNQ462/PRO790/PRO956) (AP)

Cat. Num. AAA6506037

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: WISP3; Polyclonal Antibody; ID (WNT1-inducible-signaling Pathway Protein 3; WISP-3; CCN Family Member 6; CCN6; UNQ462/PRO790/PRO956) (AP); anti-WISP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WISP3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 354

• Applicable Applications for anti-WISP3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human WISP3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-WISP3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). WISP3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-WISP3 antibody

Antibodies; Wnt Proteins

References

1. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2. Tanaka, S., et al., Gastroenterology 123(1):392-393 (2002). 3. Kleer, C.G., et al., Oncogene 21(20):3172-3180 (2002). 4. Hurvitz, J.R., et al., Nat. Genet. 23(1):94-98 (1999). 5. Pennica, D., et al., Proc. Natl. Acad. Sci. USA 95(25):14717-14722 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4507925
• NCBI Accession #: NP_003871
• NCBI GenBank Nucleotide #: NP_003871.1
• UniProt Accession #: O95389
• Molecular Weight: 39.292
• NCBI Official Full Name: cellular communication network factor 6 isoform 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP3
• UniProt Synonym Gene Names: CCN6; WISP-3
• UniProt Entry Name: WISP3_HUMAN

Uniprot Description

WISP3: Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC). PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: proteinaceous extracellular matrix; extracellular space

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion

Disease: Arthropathy, Progressive Pseudorheumatoid, Of Childhood