Human Polycystin-1 ELISA Kit | PKD1 elisa kit

Human Polycystin-1 ELISA Kit

Cat. Num. AAA764345

• Gene Names: PKD1; PBP; Pc-1; TRPP1
• Reactivity: Human
• Synonyms: Polycystin-1; Human Polycystin-1 ELISA Kit; PKD1/Polycystic kidney disease protein 1-like 1/PC1-like 1 protein/Polycystin-1L1; PKD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PKD1. No significant cross-reactivity or interference between PKD1 and analogues was observed.
• Sequence Length: 4302

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKD1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 205360962
• NCBI GeneID: 5310
• NCBI Accession #: NP_000287.3
• NCBI GenBank Nucleotide #: NM_000296.3
• Molecular Weight: 462,401 Da
• NCBI Official Full Name: polycystin-1 isoform 2
• NCBI Official Synonym Full Names: polycystin 1, transient receptor potential channel interacting
• NCBI Official Symbol: PKD1
• NCBI Official Synonym Symbols: PBP; Pc-1; TRPP1
• NCBI Protein Information: polycystin-1
• UniProt Protein Name: Polycystin-1
• Protein Family: Polycystin
• UniProt Gene Name: PKD1
• UniProt Entry Name: PKD1_HUMAN

NCBI Description

This gene encodes a member of the polycystin protein family. The encoded glycoprotein contains a large N-terminal extracellular region, multiple transmembrane domains and a cytoplasmic C-tail. It is an integral membrane protein that functions as a regulator of calcium permeable cation channels and intracellular calcium homoeostasis. It is also involved in cell-cell/matrix interactions and may modulate G-protein-coupled signal-transduction pathways. It plays a role in renal tubular development, and mutations in this gene cause autosomal dominant polycystic kidney disease type 1 (ADPKD1). ADPKD1 is characterized by the growth of fluid-filled cysts that replace normal renal tissue and result in end-stage renal failure. Splice variants encoding different isoforms have been noted for this gene. Also, six pseudogenes, closely linked in a known duplicated region on chromosome 16p, have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

PKD1: Involved in renal tubulogenesis. Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Acts as a regulator of cilium length, together with PKD2. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. May be an ion- channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1). ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000. Belongs to the polycystin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: basolateral plasma membrane; cilium; cytoplasm; Golgi membrane; integral to plasma membrane; nucleus; plasma membrane

Molecular Function: calcium channel activity; protein binding; protein domain specific binding; protein kinase binding

Biological Process: anatomical structure morphogenesis; branching morphogenesis of a tube; calcium-independent cell-matrix adhesion; cartilage development; cell cycle arrest; cell-matrix adhesion; cytoplasmic sequestering of transcription factor; detection of mechanical stimulus; embryonic placenta development; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; genitalia development; gut development; heart development; homophilic cell adhesion; in utero embryonic development; JAK-STAT cascade; kidney development; neural tube development; peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; protein export from nucleus; skin development; spinal cord development

Disease: Polycystic Kidney Disease 1