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Typical Testing Data/Standard Curve (for reference only) ()

Human squamous cell carcinoma antigen (SCCAg) ELISA Kit | SCCAg elisa kit

Human squamous cell carcinoma antigen (SCCAg) ELISA Kit

Gene Names
SART3; P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
Reactivity
Human
Synonyms
squamous cell carcinoma antigen (SCCAg); Human squamous cell carcinoma antigen (SCCAg) ELISA Kit; SCCAg elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
963
Samples
Human serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for SCCAg elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human SCCAg monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,773 Da
NCBI Official Full Name
squamous cell carcinoma antigen recognized by T-cells 3
NCBI Official Synonym Full Names
squamous cell carcinoma antigen recognized by T cells 3
NCBI Official Symbol
SART3
NCBI Official Synonym Symbols
P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
NCBI Protein Information
squamous cell carcinoma antigen recognized by T-cells 3; SART-3; hSART-3; tat-interacting protein of 110 kDa
UniProt Protein Name
Squamous cell carcinoma antigen recognized by T-cells 3
UniProt Gene Name
SART3
UniProt Synonym Gene Names
KIAA0156; TIP110; SART-3; hSART-3; Tip110
UniProt Entry Name
SART3_HUMAN

NCBI Description

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

SART3: a ubiquitously expressed protein in proliferating cells. A component of the 7SK snRNP complex composed of P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Transiently associates with the U6 and U4/U6 snRNPs during the spliceosome cycle, helping to regenerate functional U4/U6 snRNPs complexes. Expressed in the nucleus of all of malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas, adenocarcinomas, melanomas and leukemias. However, this protein is undetectable in the nucleus of nonmalignant cell lines or normal tissues, except for the testis. Expressed in the cytoplasm of all proliferating cells, including normal and malignant cells, but not in most cells of normal tissues, except for the testis and the fetal liver. Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Transcription regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; Cajal body; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus

Molecular Function: U4 snRNA binding; protein binding; U6atac snRNA binding; histone binding; nucleotide binding; U6 snRNA binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; nucleosome assembly; spliceosomal snRNP biogenesis; regulation of gene expression; cell morphogenesis; homeostasis of number of cells

Disease: Porokeratosis 3, Disseminated Superficial Actinic Type

Research Articles on SCCAg

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Product Notes

The Human SCCAg sart3 (Catalog #AAA263873) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263873 ELISA Kit recognizes Human SCCAg. It is sometimes possible for the material contained within the vial of "squamous cell carcinoma antigen (SCCAg), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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