Pituitary homeobox 3 Recombinant Protein | Pitx3 recombinant protein

Recombinant Mouse Pituitary homeobox 3

Cat. Num. AAA1036049

• Gene Names: Pitx3; ak; Ptx3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Pituitary homeobox 3; Recombinant Mouse Pituitary homeobox 3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3; Pitx3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-302aa; Full Length
• Sequence: MEFGLLGEAEARSPALSLSDAGTPHPPLPEHGCKGQEHSDSEKASASLPGGSPEDGSLKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERSQQAELCKGGFAAPLGGLVPPYEEVYPGYSYGNWPPKALAPPLAAKTFPFAFNSVNVGPLASQPVFSPPSSIAASMVPSAAAAPGTVPGPGALQGLGGAPPGLAPAAVSSGAVSCPYASAAAAAAAAASSPYVYRDPCNSSLASLRLKAKQHASFSYPAVPGPPPAANLSPCQYAVERPV
• Sequence Length: 302

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for Pitx3 recombinant protein

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the tporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.

References

Isolation of a new homeobox gene belonging to the Pitx/Rieg family expression during lens development and mapping to the aphakia region on mouse chromosome 19.Semina E.V., Reiter R.S., Murray J.C.Hum. Mol. Genet. 6:2109-2116(1997) A novel homeobox gene PITX3 is mutated in families with autosomal-dominant cataracts and ASMD.Semina E.V., Ferrell R.E., Mintz-Hittner H.A., Bitoun P., Alward W.L.M., Reiter R.S., Funkhauser C., Daack-Hirsch S., Murray J.C.Nat. Genet. 19:167-170(1998) Pitx3 regulates tyrosine hydroxylase expression in the substantia nigra and identifies a subgroup of mesencephalic dopaminergic progenitor neurons during mouse development.Maxwell S.L., Ho H.Y., Kuehner E., Zhao S., Li M.Dev. Biol. 282:467-479(2005) Induction of tyrosine hydroxylase expression by the transcription factor Pitx3.Messmer K., Remington M.P., Skidmore F., Fishman P.S.Int. J. Dev. Neurosci. 25:29-37(2007) Pitx3 controls multiple aspects of lens development.Medina-Martinez O., Shah R., Jamrich M.Dev. Dyn. 238:2193-2201(2009) Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression.Jacobs F.M., van Erp S., van der Linden A.J., von Oerthel L., Burbach J.P., Smidt M.P.Development 136:531-540(2009) Homeodomain protein Pitx3 maintains the mitotic activity of lens epithelial cells.Ho H.Y., Chang K.H., Nichols J., Li M.Mech. Dev. 126:18-29(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 6679341
• NCBI GeneID: 18742
• NCBI Accession #: NP_032878.1
• NCBI GenBank Nucleotide #: NM_008852.4
• UniProt Accession #: O35160; Q0VB03
• Molecular Weight: 33.7 kDa
• NCBI Official Full Name: pituitary homeobox 3
• NCBI Official Synonym Full Names: paired-like homeodomain transcription factor 3
• NCBI Official Symbol: Pitx3
• NCBI Official Synonym Symbols: ak; Ptx3
• NCBI Protein Information: pituitary homeobox 3
• UniProt Protein Name: Pituitary homeobox 3
• Protein Family: Pituitary homeobox
• UniProt Gene Name: Pitx3
• UniProt Entry Name: PITX3_MOUSE

Uniprot Description

PITX3: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. Defects in PITX3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: lens development in camera-type eye; lens morphogenesis in camera-type eye; locomotory behavior; midbrain development; multicellular organismal development; neuron development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of gene expression; regulation of transcription, DNA-dependent; transcription, DNA-dependent