Typical Testing Data/Standard Curve (for reference only)
Bovine Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein ELISA Kit | PIN4 elisa kit
Bovine Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This PIN4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PIN4. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY PIN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PIN4 antibody and an PIN4-HRP conjugate. The assay sample and buffer are incubated together with PIN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIN4 concentration since PIN4 from samples and PIN4-HRP conjugate compete for the anti-PIN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PIN4 from the sample, fewer sites are left to bind PIN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIN4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
Uniprot Description
PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 5.2.1.8; Mitochondrial; Isomerase; Nucleolus
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: mitochondrial matrix; cytoplasm; nucleolus; spindle; nucleus
Molecular Function: peptidyl-prolyl cis-trans isomerase activity; DNA binding; double-stranded DNA binding; bent DNA binding
Biological Process: protein peptidyl-prolyl isomerization; rRNA processing
Research Articles on PIN4
Similar Products
Product Notes
The Bovine PIN4 pin4 (Catalog #AAA740555) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740555 ELISA Kit recognizes Bovine PIN4. It is sometimes possible for the material contained within the vial of "Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
