Rat phosphoinositide-3-kinase, regulatory subunit 1 (alpha) ELISA Kit | PIK3R1 elisa kit

Rat Phosphatidylinositol 3-kinase regulatory subunit alpha, PIK3R1 ELISA Kit

Cat. Num. AAA9322648

• Gene Names: Pik3r1; PI3KA
• Reactivity: Rat
• Synonyms: phosphoinositide-3-kinase; regulatory subunit 1 (alpha); Rat Phosphatidylinositol 3-kinase regulatory subunit alpha; PIK3R1 ELISA Kit; Rat Phosphatidylinositol 3-kinase regulatory subunit alpha (PIK3R1) ELISA kit; GRB1; p85; p85-ALPHA; phosphatidylinositol 3-kinase; regulatory subunit; polypeptide 1 (p85 alpha) ; regulatory; 1; phosphatidylinositol 3-kinase-associated p-85 alpha; PIK3R1 elisa kit

• Reactivity: Rat

• Samples: Undiluted original Rat serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 0.25ng/ml-8ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PIK3R1 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of PIK3R1 (hereafter termed "analyte") in undiluted original Rat serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 6981358
• NCBI GeneID: 25513
• NCBI Accession #: NP_037137.1
• NCBI GenBank Nucleotide #: NM_013005.1
• UniProt Accession #: Q63787; O55085; P70544; Q63790
• Molecular Weight: 83,531 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: Pik3r1
• NCBI Official Synonym Symbols: PI3KA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; ptdIns-3-kinase p85-alpha; PI3-kinase p85 subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit p85-alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: Pik3r1
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
• UniProt Entry Name: P85A_RAT

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Enzyme, regulatory subunit; Kinase, lipid

Cellular Component: neuron projection; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; intercellular junction; phosphoinositide 3-kinase complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; neurotrophin TRKA receptor binding; 1-phosphatidylinositol-3-kinase activity; platelet-derived growth factor receptor binding; phosphoprotein binding; 1-phosphatidylinositol-3-kinase regulator activity; phosphoinositide 3-kinase regulator activity; receptor tyrosine kinase binding; protein kinase binding; transcription factor binding; protein phosphatase binding; ATPase binding; insulin receptor binding; calmodulin binding; insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; ubiquitin protein ligase binding; insulin receptor substrate binding; estrogen receptor binding; phosphoinositide 3-kinase binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; response to cAMP; negative regulation of heart rate; negative regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; regulation of phosphoinositide 3-kinase activity; NFAT protein import into nucleus; protein amino acid phosphorylation; positive regulation of RNA splicing; response to estradiol stimulus; response to insulin stimulus; protein transport; phosphoinositide phosphorylation; positive regulation of transcription factor import into nucleus; response to yeast; response to iron(II) ion; negative regulation of blood pressure; response to nutrient; response to fructose stimulus; aging; negative regulation of proteolysis; response to drug; protein stabilization; glucose metabolic process; response to testosterone stimulus; response to amino acid stimulus; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; positive regulation of myoblast differentiation; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; induction of apoptosis via death domain receptors; cellular response to insulin stimulus; B cell differentiation; insulin receptor signaling pathway; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; response to progesterone stimulus; positive regulation of cell migration; negative regulation of apoptosis