Typical Testing Data/Standard Curve (for reference only)
Human C-terminal propeptide of Collagen alpha-1 (II) chain ELISA Kit | PIICP elisa kit
Human C-terminal propeptide of Collagen alpha-1 (II) chain ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICTP, during the reaction, ICTP in the sample or standard competes with a fixed amount of biotin-labeled ICTP for sites on a pre-coated Monoclonal antibody specific to ICTP. Excess conjugate and unbound sample or standard are washed from the plate. Next, avidin-conjugated to Horse radish Peroxidase (HRP) is added to each microplate well and incubated. Then a T MB substrate solution is added to each well. T he enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotom etrically at awavelength of 450nm+/- 2nm. The concentration of ICTP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q13.11
Cellular Component: collagen type II; endoplasmic reticulum lumen; extracellular matrix; extracellular region
Molecular Function: extracellular matrix structural constituent conferring tensile strength; MHC class II protein binding; platelet-derived growth factor binding
Biological Process: cartilage development; collagen catabolic process; collagen fibril organization; extracellular matrix organization and biogenesis; regulation of immune response; sensory perception of sound; skeletal development; visual perception
Disease: Achondrogenesis, Type Ii; Avascular Necrosis Of Femoral Head, Primary; Czech Dysplasia; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Kniest Dysplasia; Legg-calve-perthes Disease; Osteoarthritis With Mild Chondrodysplasia; Otospondylomegaepiphyseal Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Spondyloepiphyseal Dysplasia Congenita; Spondyloepiphyseal Dysplasia, Stanescu Type; Spondyloperipheral Dysplasia; Stickler Syndrome, Type I; Stickler Syndrome, Type I, Nonsyndromic Ocular
Research Articles on PIICP
Similar Products
Product Notes
The Human PIICP col2a1 (Catalog #AAA9428188) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9428188 ELISA Kit recognizes Human PIICP. It is sometimes possible for the material contained within the vial of "C-terminal propeptide of Collagen alpha-1 (II) chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
