Polymeric Immunoglobulin Receptor (PIGR) Recombinant Protein | PIGR recombinant protein

Recombinant Polymeric Immunoglobulin Receptor (PIGR)

Cat. Num. AAA2009511

• Gene Names: Pigr; pIgA-R; RNPIGR2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Polymeric Immunoglobulin Receptor (PIGR); Recombinant Polymeric Immunoglobulin Receptor (PIGR); PIGR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LEVSQVPEFP NDTHVYTKDI GRTVTIECRF KEGNAHSKKS LCKKRGEACE VVIDSTEYVD PSYKDRAILF MKGTSRDIFY VNISHLIPSD AGLYVCQAGE GPS
• Sequence Length: 769

• Applicable Applications for PIGR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu125~Ser227 (Accession # P15083) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 501354879
• NCBI GeneID: 25046
• NCBI Accession #: NP_036855.3
• NCBI GenBank Nucleotide #: NM_012723.4
• UniProt Accession #: P15083
• Molecular Weight: 13.0kDa
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: Pigr
• NCBI Official Synonym Symbols: pIgA-R; RNPIGR2
• NCBI Protein Information: polymeric immunoglobulin receptor; poly-Ig receptor; polymeric IgA receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: Pigr
• UniProt Synonym Gene Names: PIgR
• UniProt Entry Name: PIGR_RAT

NCBI Description

contains a trinucleotide repeat-rich fragment in the 3' untranslated region that may regulate gene expression [RGD, Feb 2006]

Uniprot Description

PIGR: This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Protein type: Immunoglobulin superfamily; Receptor, misc.; Membrane protein, integral

Cellular Component: extracellular space; transport vesicle; recycling endosome membrane; extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function: polymeric immunoglobulin receptor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; retinal homeostasis; detection of chemical stimulus involved in sensory perception of bitter taste; immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor; receptor clustering