Pig Lipocalin 1 (LCN1) ELISA Kit | LCN1 elisa kit

Pig Lipocalin 1 (LCN1) ELISA Kit

Cat. Num. AAA2707550

• Gene Names: LCN1; TP; TLC; PMFA; VEGP
• Reactivity: Pig
• Synonyms: Lipocalin 1 (LCN1); Pig Lipocalin 1 (LCN1) ELISA Kit; TP; PMFA; VEGP; Tlc; Tear Prealbumin; Tear lipocalin; Protein Migrating Faster Than Albumin; Von Ebner Gland Protein; LCN1 elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipocalin 1 (LCN1).; No significant cross-reactivity or interference between Lipocalin 1 (LCN1) and analogues was observed.
• Sequence Length: 176

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.61ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipocalin 1 (LCN1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipocalin 1 (LCN1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipocalin 1 (LCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipocalin 1 (LCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipocalin 1 (LCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipocalin 1 (LCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504963
• NCBI GeneID: 3933
• NCBI GenBank Nucleotide #: NP_002288.1
• UniProt Accession #: P31025
• NCBI Official Full Name: lipocalin-1 isoform 1
• NCBI Official Synonym Full Names: lipocalin 1
• NCBI Official Symbol: LCN1
• NCBI Official Synonym Symbols: TP; TLC; PMFA; VEGP
• NCBI Protein Information: lipocalin-1
• UniProt Protein Name: Lipocalin-1
• Protein Family: Lipocalin
• UniProt Gene Name: LCN1
• UniProt Synonym Gene Names: VEGP; Tlc; TP; VEG protein
• UniProt Entry Name: LCN1_HUMAN

NCBI Description

This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]

Uniprot Description

LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region

Molecular Function: cysteine protease inhibitor activity

Biological Process: long-chain fatty acid transport; retinal homeostasis; sensory perception of taste; response to stimulus; proteolysis; transmembrane transport