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Typical Testing Data/Standard Curve (for reference only)

Pig hepatocyte growth factor, HGF ELISA Kit | HGF elisa kit

Pig hepatocyte growth factor, HGF ELISA Kit

Gene Names
HGF; SF; HGFB; HPTA; F-TCF; DFNB39
Reactivity
Pig
Synonyms
hepatocyte growth factor; HGF; Pig hepatocyte growth factor; HGF ELISA Kit; HGF elisa kit
Ordering
For Research Use Only!
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of pig CGRP. No significant cross-reactivity or interference between pig CGRP and analogues was observed.
Sequence Length
723
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
<7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HGF elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CGRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CGRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,116 Da
NCBI Official Full Name
hepatocyte growth factor isoform 3
NCBI Official Synonym Full Names
hepatocyte growth factor (hepapoietin A; scatter factor)
NCBI Official Symbol
HGF
NCBI Official Synonym Symbols
SF; HGFB; HPTA; F-TCF; DFNB39
NCBI Protein Information
hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
UniProt Protein Name
Hepatocyte growth factor
Protein Family
UniProt Gene Name
HGF
UniProt Synonym Gene Names
HPTA; SF
UniProt Entry Name
HGF_HUMAN

NCBI Description

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.2 PublicationsManual assertion based on experiment in:Ref.23

Research Articles on HGF

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Product Notes

The Pig HGF hgf (Catalog #AAA703830) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703830 ELISA Kit recognizes Pig HGF. It is sometimes possible for the material contained within the vial of "hepatocyte growth factor, HGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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