Cytochrome P450 1A2 Active Protein | CYP1A2 active protein

EZCyp™ Active Human Cytochrome P450 1A2

Cat. Num. AAA845570

• Gene Names: CYP1A2; CP12; CYPIA2; P3-450; P450(PA)
• Synonyms: Cytochrome P450 1A2; EZCyp™ Active Human Cytochrome P450 1A2; Cytochrome P450 1A; Cyt1a2; CYPIA; CYPIA2; Cyp1A2; Microsomal cytochrome P450 1A2; active CYP1A2; human cyp1A2; human CYP1A2; recombinant CYP1A; CP12; P3-450; P450 (PA); CYP1A2 active protein

• Host: Pichia pastoris co-expressing NADPH-Reductase
• Form/Format: Dry powder
• Sequence Length: 516

• Advantages of the BioVision EZCyp™ 1A2: - Co-expresses human CYP1A2 and human P450 oxidoreductase (hCPR) allowing easy reconstitution of the endogenous system.; - Useful for all the applications as a baculosome system but more cost-effective ; - Easy to handle dry powder ; - Very stable in dry form and active in multiple buffers and solvents during long incubations; - Addition of NADP+ & Glucose-6-Phosphate are not essential (but will boost activity); - After the reaction, the protein can be pelleted at relatively low speeds ; - Clean HPLC profiles for easy metabolite ID and purification ; - Highly controlled production process for lot-to-lot reproducibility
• Preparation and Storage: Stable for 1 year as supplied. Store dry powder at -20°C. Reconstitute in 100 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP1A2 will lose approximately 10% activity per week when stored at -80°C). Thaw aliquots rapidly at 37°C and place on ice until use (thawed aliquots should be used within 4 hours).

Activity

(Fig: Activity of recombinant human CYP1A2 using the Cytochrome P450 1A2 (CYP1A2) Activity Assay Kit (Fluorometric).)

Related Product Information for CYP1A2 active protein

Cytochrome P450 1A2 (CYP1A2; EC 1.14.14.1) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. CYPs are membrane-bound hemeproteins responsible for Phase I biotransformation reactions; in which lipophilic drugs and other xenobiotic compounds are converted to more hydrophilic products to facilitate excretion from the body. CYP1A2 metabolizes many antidepressants and antipsychotic drugs; some of its substrates being caffeine; acetaminophen; fluvoxamine and Haloperidol. Expression of CYP1A2 appears to be induced by various dietary constituents like cabbages; cauliflower and broccoli. EZCypTM 1A2 is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP1A2 and recombinant human P450 NADPH oxidoreductase co expressed in the same preparation.

NCBI and Uniprot Product Information

• NCBI GI #: 73915100
• NCBI GeneID: 1544
• NCBI Accession #: NP_000752.2
• NCBI GenBank Nucleotide #: NM_000761.4
• UniProt Accession #: P05177; Q16754; Q6NWU5; Q9BXX7; Q9UK49
• NCBI Official Full Name: cytochrome P450 1A2
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily A member 2
• NCBI Official Symbol: CYP1A2
• NCBI Official Synonym Symbols: CP12; CYPIA2; P3-450; P450(PA)
• NCBI Protein Information: cytochrome P450 1A2
• UniProt Protein Name: Cytochrome P450 1A2
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A2

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Lipid Metabolism - linoleic acid; Oxidoreductase; Secondary Metabolites Metabolism - caffeine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: demethylase activity; electron carrier activity; enzyme binding; heme binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: alkaloid metabolic process; drug catabolic process; drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; heterocycle metabolic process; methylation; monocarboxylic acid metabolic process; monoterpenoid metabolic process; steroid catabolic process; toxin biosynthetic process; xenobiotic metabolic process