PHF1 / PHD Finger Protein 1 Recombinant Protein | PHF1 recombinant protein

Human PHF1 / PHD Finger Protein 1 Recombinant Protein

• Gene Names: PHF1; PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: PHF1 / PHD Finger Protein 1; Human PHF1 / PHD Finger Protein 1 Recombinant Protein; PHD finger protein 1; Protein PHF1; Polycomb-like protein 1; hPCl1; PCL1; PHF1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 457

• Applicable Applications for PHF1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with CHMP1 (By similarity). Associated component of the PRC2 complex. Interacts with p53/TP53.
• Entry Function: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4505777
• NCBI GeneID: 5252
• NCBI Accession #: NP_002627.1
• NCBI GenBank Nucleotide #: NP_002627.1
• UniProt Accession #: O43189
• NCBI Official Full Name: PHD finger protein 1 isoform a
• NCBI Official Synonym Full Names: PHD finger protein 1
• NCBI Official Symbol: PHF1
• NCBI Official Synonym Symbols: PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
• NCBI Protein Information: PHD finger protein 1
• UniProt Protein Name: PHD finger protein 1
• Protein Family: PHD finger protein
• UniProt Gene Name: PHF1
• UniProt Synonym Gene Names: PCL1; Protein PHF1; hPHF1; hPCl1
• UniProt Entry Name: PHF1_HUMAN

NCBI Description

This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

PHF1: Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; microtubule organizing center; nucleus

Molecular Function: zinc ion binding; transcription factor activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; response to DNA damage stimulus; regulation of gene expression, epigenetic