PGD recombinant protein

PGD, 1-483aa, Human, His tag, E Coli

Cat. Num. AAA203633

• Gene Names: PGD; 6PGD
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PGD; 1-483aa; Human; His tag; E Coli; Phosphogluconate dehydrogenase; 6PGD; Phosphogluconate dehydrogenase 0610042A05Rik; Au019875; C78335; 6 phosphogluconate dehydrogenase; decarboxylating; OTTMuSP00000011754; RP23-249G3.4; ; PGD recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
• Sequence Length: 483

• Applicable Applications for PGD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PGD recombinant protein

PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PGD recombinant protein

Redox Proteins

References

Le TD., et al. (2010) Proc Natl Acad Sci u S A. 107 (7):3198-203. ; Tagen M., et al. (2009) J Immunol. 183 (10):6313-9.;

NCBI and Uniprot Product Information

• NCBI GI #: 40068518
• NCBI GeneID: 5226
• NCBI Accession #: NP_002622
• NCBI GenBank Nucleotide #: NM_002631.3
• UniProt Accession #: P52209; Q9BWD8; A8K2Y9; B4DQJ8
• Molecular Weight: 55.3 kDa (503aa)
• NCBI Official Full Name: 6-phosphogluconate dehydrogenase, decarboxylating isoform 1
• NCBI Official Synonym Full Names: phosphogluconate dehydrogenase
• NCBI Official Symbol: PGD
• NCBI Official Synonym Symbols: 6PGD
• NCBI Protein Information: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Protein Name: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Gene Name: PGD
• UniProt Synonym Gene Names: PGDH

NCBI Description

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

Uniprot Description

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.