Porcine Pepsinogen C ELISA Kit | PGC elisa kit

Porcine Pepsinogen C ELISA Kit

Cat. Num. AAA740488

• Reactivity: Porcine
• Synonyms: Pepsinogen C; Porcine Pepsinogen C ELISA Kit; PGC elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PGC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGC elisa kit

Principle of the Assay: PGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGC antibody and an PGC-HRP conjugate. The assay sample and buffer are incubated together with PGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGC concentration since PGC from samples and PGC-HRP conjugate compete for the anti-PGC antibody binding site. Since the number of sites is limited, as more sites are occupied by PGC from the sample, fewer sites are left to bind PGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 387015
• Molecular Weight: 34,208 Da
• NCBI Official Full Name: pepsinogen C
• UniProt Protein Name: Gastricsin
• Protein Family: Gastricsin
• UniProt Gene Name: PGC
• UniProt Entry Name: PEPC_HUMAN

Uniprot Description

PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.23.3; Protease

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular space

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis