Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-Actinin 3 (ACTN-3) Standard Curve.)
Human Alpha-Actinin 3 (ACTN-3) ELISA Kit | ACTN-3 elisa kit
Human Alpha-Actinin 3 (ACTN-3) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-Actinin 3 (ACTN-3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-Actinin 3 (ACTN-3) Standard Curve.)
Background: Actinin, alpha 3, also known as ACTN3, is a protein which in humans is encoded by the ACTN3 gene.Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments. Skeletal muscle is composed of long cylindrical cells called muscle fibers. There are two types of muscle fibers, slow twitch or muscle contraction (type I) and fast twitch (type II). Slow twitch fibers are more efficient in using oxygen to generate energy whilst fast twitch fibers are less efficient. However, fast twitch fibers fire more rapidly and generate more force.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]
Uniprot Description
ACTN3: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Belongs to the alpha-actinin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: focal adhesion; actin filament; pseudopodium; cytosol
Molecular Function: integrin binding; protein binding; protein homodimerization activity; structural constituent of muscle; calcium ion binding; actin binding
Biological Process: regulation of apoptosis; focal adhesion formation; muscle filament sliding
Research Articles on ACTN-3
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Product Notes
The Human ACTN-3 actn3 (Catalog #AAA9714565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714565 ELISA Kit recognizes Human ACTN-3. It is sometimes possible for the material contained within the vial of "Alpha-Actinin 3 (ACTN-3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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