Western Blot (WB)
Perfringolysin O (pfo) Recombinant Protein | pfo recombinant protein
Recombinant Clostridium perfringens Perfringolysin O (pfo)
Reactivity
Clostridium perfringens (strain 13 / Type A)
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Perfringolysin O (pfo); Recombinant Clostridium perfringens Perfringolysin O (pfo); Theta-toxin; pfo recombinant protein
Host
E Coli
Reactivity
Clostridium perfringens (strain 13 / Type A)
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
Tris-based buffer, 50% glycerol
Sequence Positions
Full Length of Mature Protein, 29-500aa
Sequence
KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN
SDS-PAGE MW
67.92 kDa
Target name
pfo
Tag Info
N-terminal 6xHis-SUMO-tagged
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Western Blot (WB)
Western Blot (WB)
Related Product Information for pfo recombinant protein
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
perfringolysin O
UniProt Protein Name
Perfringolysin O
Protein Family
UniProt Gene Name
pfo
UniProt Synonym Gene Names
pfoA; pfoR
Uniprot Description
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.
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Product Notes
The pfo pfo (Catalog #AAA9423845) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length of Mature Protein, 29-500aa. The Recombinant Clostridium perfringens Perfringolysin O (pfo) reacts with Clostridium perfringens (strain 13 / Type A) and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: KDITDKNQSI DSGISSLSYN RNEVLASNGD KIESFVPKEG KKTGNKFIVV ERQKRSLTTS PVDISIIDSV NDRTYPGALQ LADKAFVENR PTILMVKRKP ININIDLPGL KGENSIKVDD PTYGKVSGAI DELVSKWNEK YSSTHTLPAR TQYSESMVYS KSQISSALNV NAKVLENSLG VDFNAVANNE KKVMILAYKQ IFYTVSADLP KNPSDLFDDS VTFNDLKQKG VSNEAPPLMV SNVAYGRTIY VKLETTSSSK DVQAAFKALI KNTDIKNSQQ YKDIYENSSF TAVVLGGDAQ EHNKVVTKDF DEIRKVIKDN ATFSTKNPAY PISYTSVFLK DNSVAAVHNK TDYIETTSTE YSKGKINLDH SGAYVAQFEV AWDEVSYDKE GNEVLTHKTW DGNYQDKTAH YSTVIPLEAN ARNIRIKARE CTGLAWEWWR DVISEYDVPL TNNINVSIWG TTLYPGSSIT YN. It is sometimes possible for the material contained within the vial of "Perfringolysin O (pfo), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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