Rabbit anti-Human GATA4 Polyclonal Antibody | anti-GATA4 antibody

GATA4, CT (GATA Binding Protein 4, GATA-binding Factor 4, Transcription Factor GATA-4) (MaxLight 490)

Cat. Num. AAA6477575

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GATA4; Polyclonal Antibody; CT (GATA Binding Protein 4; GATA-binding Factor 4; Transcription Factor GATA-4) (MaxLight 490); anti-GATA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GATA4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GATA4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GATA4 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-GATA4 Antibody (C-term) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(GATA4 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GATA4 antibody

Antibodies; Transcription Factors

References

1. Rajagopal, S.K., J. Mol. Cell. Cardiol. 43(6), 677-685 (2007). 2. Philips, A.S., J. Biol. Chem. 282(34), 24915-24927 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 33188461
• NCBI GeneID: 2626
• NCBI Accession #: NP_002043
• NCBI GenBank Nucleotide #: NM_002052
• UniProt Accession #: P43694; Q3MJ45; Q5IFM8; B7ZKX0; B7ZKZ4
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: transcription factor GATA-4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4; GATA-binding factor 4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; transcription activator binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; negative regulation of autophagy; positive regulation of transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; regulation of transcription, DNA-dependent; cell-cell signaling; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to estrogen stimulus; response to mechanical stimulus; endoderm formation; positive regulation of cardioblast differentiation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; blood coagulation; endoderm development

Disease: Ventricular Septal Defect 1; Tetralogy Of Fallot; Atrial Septal Defect 2; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease