PFK2 / PFKFB3 recombinant protein

Recombinant Human PFK2 / PFKFB3 Protein (His & GST tag)

Cat. Num. AAA2545030

• Gene Names: PFKFB3; PFK2; IPFK2; iPFK-2
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: PFK2 / PFKFB3; Recombinant Human PFK2 / PFKFB3 Protein (His & GST tag); RP11-298K24.3; IPFK2; PFK2; PFK2 / PFKFB3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% glycerol, 0.3mM DTT
• Sequence Length: 534

• Application Notes: The recombinant human PFKFB3 /GST chimera consists of 757 amino acids and has a calculated molecular mass of 87.4 KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PFK2 / PFKFB3 recombinant protein

Background: Fructose-2, 6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2, 6-bisphosphate (Fru-2, 6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase / fructose-2, 6-bisphosphatase (PFK-2 / FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2, 6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase / fructose-2, 6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions.

Description: A DNA sequence encoding the human PFKFB3 isoform 1 (Q16875-1) (Met 1-His 520) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 544063393
• NCBI GeneID: 5209
• NCBI Accession #: NP_001269559.1
• NCBI GenBank Nucleotide #: NM_001282630.2
• UniProt Accession #: Q16875; O43622; O75902; Q5VX15; Q5VX18; Q5VX19; B7Z955
• Molecular Weight: 60,602 Da
• NCBI Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 3
• NCBI Official Synonym Full Names: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
• NCBI Official Symbol: PFKFB3
• NCBI Official Synonym Symbols: PFK2; IPFK2; iPFK-2
• NCBI Protein Information: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
• UniProt Protein Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
• UniProt Gene Name: PFKFB3
• UniProt Synonym Gene Names: 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3

NCBI Description

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]

Uniprot Description

PFKFB3: Synthesis and degradation of fructose 2,6-bisphosphate. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; EC 3.1.3.46; Kinase, other; Motility/polarity/chemotaxis; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: 6-phosphofructo-2-kinase activity